Mingxing TIAN
Professional Title: Associate Professor
Education: PhD
Supervisor Category: Master Supervisor
Research Fields: the Pathogenic Mechanism of Brucella
1. Contact Information
Tel: +86-21-34293412
Mobile: +86-18817519805
E-mail: mxtian@shvri.ac.cn; tianmx530@126.com
2. Research work
(1) Identification of novel Brucella genes related to virulence and elucidation of their functions.
(2) Screening novel Brucella secreted effectors and elucidating their roles in Brucella intracellular trafficking and interaction with the host immune system.
(3) Development of new vaccines and diagnostic technologies for brucellosis.
3. Awards and Honors
Advanced and Civilized Employees of SHVRI (2016, 2023)
Excellent Post-Doc of CAAS (2016).
Award of Boehringer-Inglheim Scholarship (2014).
Outstanding Graduate of Sichuan Agricultural University (2011)
Outstanding Undergraduate of Sichuan Agricultural University (2008)
4. Grant Support
National Natural Science Foundation of China (2021-2024), Grant Number: 32072828
National Natural Science Foundation of China (2017-2019), Grant Number: 31602070
Subtopics of National key research and development program (2021-2025), Grant Number: 2021YFD1800403
Subtopics of National key research and development program (2018-2020), Grant Number: 2018YFD0500503
Shanghai Sailing Program (2015-2019), Grant/Award Number: 16YF1414600
National Basic Fund for Research Institutes, which is supported by Chinese Academy of Agricultural Sciences (2016-2018, 2019), Grant Number: 2016JB06 and 2019JB02
A First-class General Financial Grant from the China Postdoctoral Science Foundation (2015-2016), Grant Number: 2015M570184.
5. Selected Publications
[1]. Yin Y, Fang T, Lian Z, Zuo D, Hu H, Zhang G, Ding C, Tian M#, Yu S# (2023) Erythronate utilization activates VdtR regulating its metabolism to promote Brucella proliferation, inducing abortion in mice. Microbiol Spectr 11: e0207423.
[2]. Abdelgawad HA, Lian Z, Yin Y, Fang T, Tian M#, Yu S# (2023) Characterization of Brucella abortus Mutant A19mut2, a Potential DIVA Vaccine Candidate with a Modification on Lipopolysaccharide. Vaccines (Basel) 11.
[3]. Zuo D, Yin Y, Fang T, Jiang H, Ding J, Hu H, Wang S, Qi J, Tian M#, Yu S# (2022) A homolog of low molecular weight protein tyrosine phosphatase isolated from Brucella melitensis displays an acidic dual specific phosphatase activity, nonessential for bacterial resistance to bactericidal factors and virulence. Comp Immunol Microbiol Infect Dis 90-91: 101904.
[4]. Tian M#. Li Z#, Qu J, Fang T, Yin Y, Zuo D, Abdelgawad HA, Hu H, Wang S, Qi J, Wang G, Yu S (2022) The novel LysR-family transcriptional regulator BvtR is involved in the resistance of Brucella abortus to nitrosative stress, detergents and virulence through the genetic regulation of diverse pathways. Vet Microbiol 267: 109393.
[5]. Cheng Z, Li Z, Yin Y, Lian Z, Abdelgawad HA, Hu H, Guan X, Zuo D, Cai Y, Ding C, Wang S, Li T, Qi J, Tian M*, Yu S* (2021) Characteristics of Brucella abortus vaccine strain A19 reveals its potential mechanism of attenuated virulence. Vet Microbiol 254: 109007.
[6]. Tian M#, Song M#, Yin Y, Lian Z, Li Z, Hu H, Guan X, Cai Y, Ding C, Wang S, Li T, Qi J, Yu S (2020) Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis. Comp Immunol Microbiol Infect Dis 70: 101462.
[7]. Tian M#, Yin Y#, Lian Z, Li Z, Song M, Hu H, Guan X, Ding C, Wang S, Li T, Qi J, Yu S (2020) A rough Brucella mutant induced macrophage death depends on secretion activity of T4SS, but not on cellular Txnip- and Caspase-2-mediated signaling pathway. Vet Microbiol 244: 108648.
[8]. Tian M, Qu J, Li P, Bao Y, Liu J, Ding C, Wang S, Li T, Qi J, Yu S (2019) Identification of novel genes essential for Brucella abortus to establish infection by signature-tagged mutagenesis. Vet Microbiol 230: 130-137.
[9]. Tian M#, Lian Z#, Bao Y, Bao S, Yin Y, Li P, Ding C, Wang S, Li T, Qi J, Wang X, Yu S (2019) Identification of a novel, small, conserved hypothetical protein involved in Brucella abortus virulence by modifying the expression of multiple genes. Transbound Emerg Dis 66: 349-362.
[10]. Tian M#, Bao Y#, Li P, Hu H, Ding C, Wang S, Li T, Qi J, Wang X, Yu S (2018) The putative amino acid ABC transporter substrate-binding protein AapJ2 is necessary for Brucella virulence at the early stage of infection in a mouse model. Vet Res 49 (1): 32.
[11]. Li P#, Tian M#, Bao Y, Hu H, Liu J, Yin Y, Ding C, Wang S, Yu S (2017) Brucella rough mutant induce macrophage death via activating IRE1alpha pathway of endoplasmic reticulum stress by enhanced T4SS secretion. Front Cell Infect Microbiol 7: 422.
[12]. Tian M, Qu J, Bao Y, Gao J, Liu J, Wang S, Sun Y, Ding C, Yu S (2016) Construction of pTM series plasmids for gene expression in Brucella species. J Microbiol Methods 123: 18-23.
[13]. Gao J#, Tian M#, Bao Y, Li P, Liu J, Ding C, Wang S, Li T, Yu S (2016) Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse. Vet Res 47 (1): 87.
[14]. Tian M, Qu J, Han X, Ding C, Wang S, Peng D, Yu S (2014) Mechanism of Asp24 upregulation in Brucella abortus rough mutant with a disrupted O-antigen export system and effect of Asp24 in bacterial intracellular survival. Infect Immun 82 (7): 2840-2850.
[15]. Tian M, Qu J, Han X, Zhang M, Ding C, Ding J, Chen G, Yu S (2013) Microarray-based identification of differentially expressed genes in intracellular Brucella abortus within RAW264.7 cells. PLoS One 8 (8): e67014.