Zejun LI

Professional Title: Professor

Education: PhD

Supervisor Category: Doctoral Supervisor

Research Fields: Research and Development of Novel Vaccines and Diagnostic Reagents for Poultry

1. Introduction

Li Zejun, Professor, Doctor, Doctoral Supervisor, and Chief Scientist of the team. He currently serves as the Deputy Director of Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. He is a member of the National Biosafety Expert Committee of the National Pathogenic Microorganism Laboratory, the Chairman of the Veterinary Public Health Branch of the Chinese Association of Animal Science and Veterinary Medicine, the Chairman of the Livestock Veterinary Immunology Committee of the Shanghai Immunology Society, and an expert in the Veterinary Drug Review Expert Database. He has been selected as a national leading figure in scientific and technological innovation, a national outstanding agricultural scientific research talent, a young and middle-aged leading figure in scientific and technological innovation of the Ministry of Science and Technology’s “Innovative Talents Promotion Program,” and a leading talent in Shanghai. He has won a second prize of the National Natural Science Award and was awarded the title of “National Most Beautiful Science and Technology Worker” and the Shanghai May 1st Labor Medal in 2015.

He has made significant contributions to the field of poultry health. He has successfully led over 20 national and provincial-level projects. Additionally, he has mentored and supervised more than 40 master's and doctoral students. His research findings have been published in prestigious domestic and international journals, such as PNAS and JVI, with a total of over 100 papers, including more than 50 SCI papers. His work has been widely recognized and cited by others, with over 900 citations. Furthermore, he has authored two monographs, obtained more than 10 national invention patents, and received two certificates for the registration of new veterinary drugs, as well as one agriculture industry standard certification.

2. Contact Information

Telephone：021-34293446

Email：lizejun@shvri.ac.cn

3. Representative Publications

(1). Bao D, Lu C, Ma T, Xu G, Mao Y, Xin L, Niu S, Wu Z, Li X, Teng Q, Li Z*, Liu Q*. Hydrophobic Residues at the Intracellular Domain of the M2 Protein Play an Important Role in Budding and Membrane Integrity of Influenza Virus J Virol.2022 May 11; 96(9): e0037322.  

(2). Yan D, Li X, Wang Z, Liu X, Dong X, Fu, R, Su X, Xu B, Teng Q, Yuan C, Zhang Z, Liu Q*, Li Z*. The emergence of a disease caused by a mosquito origin Cluster 3.2 Tembusu virus in chickens in China.Vet Microbiol.2022 Sep:272:109500.  

(3). Yan D, Wang B, Shi Y, Ni X, Wu X, Li X, Liu X, Wang H, Su X, Teng Q, Yang J, Liu Q*, Li Z*. A Single Mutation at Position 120 in the Envelope Protein Attenuates Tembusu Virus in Ducks.Viruses. 2022 Feb 22;14(3):447. doi: 10.3390/v14030447.

(4). Yan W, Cui H, Engelsma M, Beerens N, van Oers MM, de Jong MCM, Li X, Liu Q, Yang J, Teng Q*, Li Z*. Molecular and Antigenic Characterization of Avian H9N2 Viruses in Southern China.Microbiol Spectr. 2022 Feb 23;10(1):e0082221.

(5). Bao D, Xue R, Zhang M, Lu C, Ma T, Ren C, Zhang T, Yang J, Teng Q, Li X, Li Z*, Liu Q*. N-Linked Glycosylation Plays an Important Role in Budding of Neuraminidase Protein and Virulence of Influenza Viruses. J Virol. 2021 Jan 13;95(3):e02042-20.

(6). Yang J*, Zhang P, Huang M, Qiao S, Liu Q, Chen H, Teng Q, Li X, Zhang Z, Yan D, Sun H, Li Z*. Key Amino Acids of M1-41 and M2-27 Determine Growth and Pathogenicity of Chimeric H17 Bat Influenza Virus in Cells and in Mice. J Virol. 2021 Sep 9;95(19):e0101921.

(7). Ma T, Nagy A, Xu G, Xin L, Bao D, Lu C, Niu S, Wu Z, Ren C, Zhang T, Yang J, Teng Q, Li X, Li Z*, Liu Q*. RNA-Seq Analysis of Influenza A Virus-Induced Transcriptional Changes in Mice Lung and Its Possible Implications for the Virus Pathogenicity in Mice. Viruses. 2021 Oct 8;13(10):2031. doi: 10.3390/v13102031.

(8). Yan D, Shi Y, Wang H, Li G, Li X, Wang B, Su X, Wang J, Teng Q, Yang J, Chen H, Liu Q, Ma W, Li Z*. A Single Mutation at Position 156 in the Envelope Protein of Tembusu Virus Is Responsible for Virus Tissue Tropism and Transmissibility in Ducks. J Virol. 2018 Aug 16;92(17):e00427-18.

(9). Zhao K*, Rong G, Teng Q, Li X, Lan H, Yu L, Yu S, Jin Z, Chen G, Li Z*.Dendrigraft poly-L-lysines delivery of DNA vaccine effectively enhances the immunogenic responses against H9N2 avian influenza virus infection in chickens.Nanomedicine. 2020 Jul;27:102209.

(10). Teng Q, Xu D, Shen W, Liu Q, Rong G, Li X, Yan L, Yang J, Chen H, Yu H, Ma W*, Li Z*. A Single Mutation at Position 190 in Hemagglutinin Enhances Binding Affinity for Human Type Sialic Acid Receptor and Replication of H9N2 Avian Influenza Virus in Mice. J Virol. 2016 Oct 14;90(21):9806-9825.

4. Granted National Invention Patents and Standards

(1). MDCK cell line stable expressing highly pathogenic avian influenza virus HA protein and its application, 2013, Chinese Patent ZL201010106774.4

(2).  Monoclonal antibody against Duck Tembusu virus, hybridoma cell line, and its application, 2012, Chinese Patent ZL201210030412.0

(3).  Duck Tembusu virus attenuated strain and its application, 2015, Chinese Patent L201210279232.6

(4).  Reagent kit for distinguishing virulent strain of Duck Tembusu virus and attenuated vaccine strain and its application, 2016, Chinese Patent ZL201410142794.5

(5).  Recombinant protein of the third structure domain of Duck Tembusu virus E protein and its application, 2017, Chinese Patent ZL201410067680.9

(6).  Monoclonal antibody against Duck Tembusu virus, hybridoma cell line, and application thereof, United States Patent US 9,500,652 B2

(7).  Cloned attenuated strain of Duck Tembusu virus and its preparation method and application, 2019, Chinese Patent ZL201410283544.3

(8).  Monoclonal antibody against H9 subtype avian influenza virus, hybridoma cell line, and application thereof, 2019, Chinese Patent ZL2015108235758

(9).  “Duck Tembusu virus disease diagnosis technique,” Agricultural Industry Standard (NY/T 3233-2018), published on May 7, 2018, implemented on September 1, 2018.

(10).  Duck Hepatitis Virus and its vaccine, reagent kit, 2014, Chinese Patent ZL201010601050.7

(11).  Agricultural industry standard: “Technical Guidelines for Diagnosis of Duck Tembusu Virus Disease” (NY/T 3233-2018), 2018, ranked first.

5. Rewards and Achievements

(1). National Natural Science Second Prize, Research on the Evolution, Cross-Species Infection, and Pathogenic Mechanisms of Avian Influenza Virus, ranked fifth, Certificate Number: 2013-Z-105-2-04-R05, 2013.

(2).  National New Veterinary Drug Registration Certificate, Duck Tembusu Virus Live Vaccine (Strain FX2010-180P), Certificate Number: (2018) New Veterinary Drug Certificate No. 41, 2018.

(3).  National New Veterinary Drug Registration Certificate, Duck Tembusu Virus ELISA Antibody Test Kit, Certificate Number: (2021) New Veterinary Drug Certificate No. 45, 2021.