Fei GAO
Professional Title: Professor
Education: PhD
Supervisor Category: Doctoral Supervisor
Research Fields: Analysis of replication and transcription mechanism of Artervirus , innovation and development of novel genetic engineering live vector vaccine with multi-conjugate and multivalent characteristics
1. Introduction
Dr. Gao Fei is a researcher, doctoral supervisor, and the executive chief of the Research Team for the Prevention and Control of Porcine Reproductive and Respiratory Syndrome (PRRS). She has been actively involved in the research on the replication and transcription mechanism of arterivirus and the development of novel gene engineering vaccines with multiple antigens. She has received four research and academic awards as the first author. She has led or participated in 12 research projects, including the key research and development project in the 14th Five-Year Plan (2021YFD1801401), the National Key Research and Development Program in the 13th Five-Year Plan - Key International S&T Innovation Cooperation Project (2016YFE0112500), the Horizon 2020 Project of the European Union (SAPHIR-633184) Workpackage3, National Natural Science Foundation projects, Shanghai Natural Science Foundation projects, Guangdong Province Key Special Project for African Swine Fever Emergency Prevention and Control, with a cumulative total funding of 21.5 million RMB. She has also participated as a key member in 11 projects, including National Natural Science Foundation Key Projects, ASFV research projects, EU Seventh Framework Program projects, and Technology Support Program projects. She has supervised the training of seven master’s students. She has published over 80 papers in domestic and international academic journals such as the,,, and, with 16 of them being SCI papers as the first or corresponding author. She has published one monograph and obtained 12 national invention patents, with 6 additional patents currently under application. She has also obtained one certificate for transgenic safety issued by the Ministry of Agriculture and one software copyright registration certificate. Her research achievements have been partially transformed into practical applications. She has been selected as an outstanding talent in the Minhang Chunshen Pyramid Talent Plan, “Science and Technology Innovation Talent” in Minhang District, and has won the Outstanding Young Talent Award of the“Chinese Academy of Agricultural Sciences-Dabeinong Talent Award”in 2022.
2. Contact Information
Tel: 021-34293773; 13917165815
Email: feigao@shvri.ac.cn
3. Representative Works
(1). Fei Gao#, Yifeng Jiang#, Guoxin Li, Liwei Li, Yujiao Zhang, Lingxue Yu, Hao Zheng, Wu Tong, Yanjun Zhou, Changlong Liu, Tongling Shan, Hai Yu, Ning Kong, Pengfei Chen, Guangzhi Tong*. Evaluation of immune efficacy of recombinant PRRSV vectored vaccine rPRRSV-E2 in piglets with maternal derived antibodies. Veterinary Microbiology 248 (2020) 108833.
(2). Fei Gao#, Yifeng Jiang#, Guoxin Li, Yujiao Zhang, Kuan Zhao, Haojie Zhu, Liwei Li, Lingxue Yu, Hao Zheng, Yanjun Zhou, Wu Tong, Guangzhi Tong*. Immune duration of a recombinant PRRSV vaccine expressing E2 of CSFV. Vaccine 38(2020) 7956-7962.
(3). Fei Gao, Yifeng Jiang, Guoxin Li, Yanjun Zhou, Lingxue Yu, Liwei Li, Wu Tong, Hao Zheng, Yujiao Zhang, Hai Yu, Tongling Shan, Shen Yang, Huan Liu, Kuan Zhao, Guangzhi Tong*. Porcine reproductive and respiratory syndrome virus expressing E2 of classical swine fever virus protects pigs from a lethal challenge of highly- pathogenic PRRSV and CSFV. Vaccine 36 (2018) 3269-3277.
(4). Yujiao Zhang, Kuan Zhang, Hao Zheng, Changlong Liu, Yifeng Jiang, Nannan Du, Liwei Li, Guoxin Li, Lingxue Yu, Yanjun Zhou, Wu Tong, Kuan Zhao, Guangzhi Tong, Fei Gao*. Development of a monoclonal antibody against porcine CD163 SRCT5 domain which partially blocks infection of PRRSV. Frontiers in veterinary science. doi:10.3389/ fvets.2020.597843.
(5). Jingjing Xu, Xuefei Chen, Jiqiang Wu, Hao Zheng, Wu Tong, Xiaoyong Chen, Chao Ye, Yuting Liu, Haojie Zhu, Xinfeng Fu, Yifeng Jiang, Ning Kong, Guangzhi Tong, Fei Gao*, Guoxin Li*. Pseudorabies virus pUL16 assists the nuclear import of VP26 through protein-protein interaction. Veterinary Microbiology 257(2021)109080.
(6). Yunlei Cao, Dongmei Han, Yujiao Zhang, Kuan Zhang, Nannan Du, Wu Tong, Guoxin Li, Hao Zheng, Changlong Liu, Fei Gao*, Guangzhi Tong*. Identification of one novel epitope targeting p54 protein of African swine fever virus using monoclonal antibody and development of a capable ELISA. Research in Veterinary Science 141(2021) 19-25.
(7). Liwei Li#, Jinxia Chen#, Zhengda Cao, Yunlei Cao, Ziqiang Guo, Wu Tong, Yanjun Zhou, Guoxin Li, Yifeng Jiang, Changlong Liu, Lingxue Yu, Sina Qiao, Jiachen Liu, Guangzhi Tong*, Fei Gao*. Recombinant bivalent live vectored vaccine against CSF and HP-PRRS revealed adequate heterogenous protection against NADC30-like strain. Frontiers in microbiology, doi:10.3389/fmicb.2021.822749.
(8). Jingjing Xu, Xuefei Chen, Yuting Liu, Xinling Fu, Hao Zheng, Wu Tong, Guangzhi Tong, Fei Gao*, Guoxin Li*. LRPRRC inhibit the proliferation of Pseudorabies Virus by interacting with UL16 protein. Veterinary Microbiology. S0378-1135(21)00350-3. http://doi.org/10.1016/j.vetmic.2021.109327.
(9). Yifeng Jiang, Guoxin Li, Lingxue Yu, Liwei Li, Yujiao Zhang, Yanjun Zhou, Wu Tong, Changlong Liu, Fei Gao*, Guangzhi Tong*. Genetic Diversity of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) From 1996 to 2017 in China. Frontiers in Microbiology. 24 April 2020. doi: 10.3389/fmicb. 2020. 00618.
(10). Zehui Qu#, Fei Gao#, Liwei Li, Yujiao Zhang, Yifeng Jiang, Lingxue Yu, Yanjun Zhou, Hao Zheng, Wu Tong, Guoxin Li, Guangzhi Tong*. Label-free quantitative proteomic analysis of differentially expressed membrane proteins of pulmonary alveolar macrophage infected with highly pathogenic porcine reproductive and respiratory syndrome virus and its attenuated strain. Proteomics. 2017 Dec; 17(23-24). doi: 10.1002/pmic.201700101 Epub 2017 Nov 24.
4. Granted Patents
(1). “A reverse genetic manipulation method based on the redistribution of transcriptional regulatory sequences and its application”, China National Intellectual Property Administration, Invention Patent, ZL202110882874.4
(2). “Preparation method, epitope identification, and application of monoclonal antibody against pig CD163 protein”, China National Intellectual Property Administration, Invention Patent, ZL202010629062.4, 2020
(3). “Preparation method, epitope identification, and application of monoclonal antibody against African Swine Fever virus p54 protein”, China National Intellectual Property Administration, Invention Patent, ZL202011556967.X, May 17, 2022
(4). “Construction method and application of PRRSV recombinant plasmid expressing sea kidney or firefly luciferase gene”, China National Intellectual Property Administration, Invention Patent, ZL201510069182.2, 2015
(5). “Construction method and application of recombinant PRRS virus gene engineering vaccine expressing swine fever virus E2 protein”, China National Intellectual Property Administration, Invention Patent, ZL201410801939.8, 2014
(6). “Preparation and application of antigenic epitopes and antibodies against swine fever virus E2 protein”, China National Intellectual Property Administration, Invention Patent, ZL201710855981.1, 2017
(7). “Infectious clone plasmid of Pseudo Rabies Virus JS-2012 strain, construction method, and application”, China National Intellectual Property Administration, Invention Patent, ZL201710118726.9, 2017
(8). “Gene-deleted attenuated strain of Pseudo Rabies Virus and its preparation method and application”, China National Intellectual Property Administration, Invention Patent, ZL20141000265, 2014
(9). “GM-CSF recombinant PRRSV attenuated vaccine strain and its preparation method and application”, China National Intellectual Property Administration, Invention Patent, ZL201410056557.7, 2014
(10). “Recombinant vesicular stomatitis virus and its construction method and application”, China National Intellectual Property Administration, Invention Patent, ZL201010115505.4, 2010
(11). “Highly pathogenic porcine reproductive and respiratory syndrome virus recombinant plasmid and genetic engineering vaccine”, China National Intellectual Property Administration, Invention Patent, Patent Number: ZL200710172364.8, 2007
(12). “Optimization software for TaqMan real-time fluorescence quantitative PCR reaction system for African Swine Fever”, Copyright owner: Gao Fei, May 5, 2021
5. GMO Safety Certificate
“Recombinant Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Vaccine rPRRSV-E2 Expressing Swine Fever Virus E2 Protein”, Agricultural Genetically Modified Organism Safety Certificate (Production and Application) of the People’s Republic of China, Certificate No. Nong Ji An Zheng Zi (2019) 022
6. Rewards
(1). Chinese Association of Animal Science and Veterinary Medicine, BI “Porcine Blue Ear Disease” Research Progress Award, Gao Fei, 2018
(2). Recombinant Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) expressing Classical Swine Fever Virus (CSFV) E2 protein protects piglets from highly pathogenic PRRSV and CSFV attacks, “2017 PRRS Prevention and Control Technology Seminar Outstanding Paper Award”, Gao Fei, Jiang Yifeng, Li Guoxin, Li Liwei, Yu Lingxue, Zhou Yanjun, Zhang Yujiao, Tong Guangzhi, 2017
(3). Porcine reproductive and respiratory syndrome virus expressing E2 of classical swine fever virus protects pigs from a lethal challenge of highly-pathogenic PRRSV and CSFV, “Rui Pu Cup” Outstanding Paper Award of the Bioproducts Division of the Chinese Association of Animal Science and Veterinary Medicine 2018 Annual Conference, Gao Fei, Jiang Yifeng, Li Guoxin, Zhou Yanjun, Yu Lingxue, Li Liwei, Tong Wu, Zheng Hao, Zhang Yujiao, Yu Hai, Shan Tongling, Yang Xin, Liu Huan, Zhao Kuan, Tong Guangzhi, 2018
(4). Creation of recombinant PRRSV vaccine expressing CSFV E2 protein, 2019 Shanghai Veterinary Medicine Association Outstanding Paper Award, Gao Fei, Jiang Yifeng, Li Guoxin, Zhou Yanjun, Yu Lingxue, Li Liwei, Tong Wu, Zheng Hao, Zhang Yujiao, Yu Hai, Shan Tongling, Yang Xin, Liu Huan, Zhao Kuan, Tong Guangzhi, 2019
(5). Research on the use of highly pathogenic Porcine Reproductive and Respiratory Syndrome Virus attenuated vaccine strain as an expression vector of exogenous genes in live virus, 2022 Shanghai Veterinary Medicine Association Outstanding Paper Award, Chen Jinxia, Zhang Kuan, Zhang Yujiao, Du Nannan, Zheng Haihong, Li Liwei, Zhou Yanjun, Yu Lingxue, Tong Wu, Zheng Hao, Tong Guangzhi, Gao Fei, 2022