Ying LIAO
Professional Title: Professor
Education: PhD
Supervisor Category: Doctoral Supervisor
Research Fields: Animal coronaviruses and avian viruses
1. Introduction
My primary research focus revolves around elucidating various aspects of the virus life cycle, host antiviral responses, and pathogenesis, with a concurrent effort to develop diagnostic reagents and antiviral therapeutics. Specifically, our investigations center on coronaviruses, paramyxoviruses, and other avian viruses. We delve into a range of topics including virus tropism and transmission, the complete virus life cycle encompassing entry, replication complex assembly, viral protein translation, and virion release. I also explore ER and mitochondria stress responses, innate immune reactions, diagnostic methodologies, antiviral therapies, and vaccine development, among other areas. My ultimate aim is to achieve a comprehensive understanding of the intricate interplay between these viruses and the host cell during infection, thereby contributing to the advancement of antiviral drugs and vaccines. Notably, I have spearheaded the construction of a reverse genetic system for avian coronavirus IBV, facilitating the development of virus vector-based vaccines. Through these research endeavors, I have published over 60 papers in academic journals such as PLOS Pathogens, Journal of Virology, Cell Death & Disease, Journal of Immunology, Virology, Viruses, Veterinary Research, Journal of General Virology, Oncotarget, FEBS Letters, and Biochemical and Biophysical Research Communications (BBRC). Additionally, I have supervised the research work of nearly twenty PhD and Master graduate students.
2. Contact Information
Cell phone: 13331872602
Email: liaoying@shvri.ac.cn
3. Education
BSc, East China Normal University, China (1993-1997)
PhD, Nanyang Technological University, Singapore (2002-2007)
4. Professional Experience
Team leader, Avian viral infectious diseases team, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China (2021-present)
Professor, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China (2014-present)
Research Fellow, School of Biological Sciences, Nanyang Technological University, Singapore (2007-2009, 2010-2014)
Research Fellow, Institute of Molecular and Cell Biology, A*STAR, Singapore (2009-2010)
5. Awarded project proposals
1) National Natural Science foundation of China (Grant No. 32372999): " Mechanism study of coronavirus targeting nucleocytoplasmic transport system to antagonize host immune response", ¥ 500,000, 2024.1-2027.12;
2) Shanghai Natural Science Foundation project (Grant No. 23ZR1477000): " Mechanism study of coronavirus E protein promoting inflammatory response, ¥ 200,000, 2023.04-2026.03;
3) National Key Research and Development Program (Grant No. 2021YFD180110): “Mechanism and function study of immune escape by animal coronaviruses”.¥ 2,000,000, 2022.1-2024.12;
4) National Natural Science foundation of China (Grant No. 32172834): “The mechanism study of host translation shutoff induced by coronavirus endonuclease nsp15”, ¥ 590,000, 2022.01-2025.12;
5) National Natural Science foundation of China (Grant No. 31772724): “Characterization of stress granules as antiviral hub for innate immune signaling”, ¥ 580,000, 2017.01-2021.12;
6) National Key Research and Development Program (Grant No. 2017YFD0500802): “Development and application of goose and duck vaccines and immunostimulants”, ¥ 4,830,000, 2017.07-2021.06;
7) Central Public-interest Scientific Institution Basal Research Fund (2019JB03): “Mechanistic study of delayed interferon response by infectious bronchitis virus”, ¥ 200,000, 2019.01-2021.12;
8) Elite Youth Program of Chinese Academy of Agricultural Sciences. ¥ 3,000,000, 2015.01-2018.12;
9) The Open Project Program of Jiangsu Key Laboratory of Zoonosis (R2007): “Coronavirus infection and prevention: Antagonizing to innate immune response by coronavirus”, ¥ 50,000, 2020.02-2022.05;
10) Central Public-interest Scientific Institution Basal Research Fund (2017): “The entry and uncoating of infectious bronchitis virus”, ¥ 150,000, 2017.01-2017.12;
11) Natural Science foundation of Shanghai (15ZR14496600): “The crosstalk of ER stress response and apoptosis triggered by Newcastle Disease Virus infection”, ¥ 100,000, 2015.01-2017.12.
6. Publications
1) Huan Wang#, Huiyu Liao, Yingjie Sun, Lei Tan, Cuiping Song, Xusheng Qiu, Chan Ding, Ying Liao#*. Classification, Replication and Transcription of Nidovirales . Frontiers in Microbiology. 2023. Nov 06. Accepted. DOI: 10.3389/fmicb.2023.1291761. (*Corresponding authors)
2) Xiao Yuan#, Xiaoman Zhang#, Huan Wang, Xiang Mao, Yingjie Sun, Lei Tan, Cuiping Song, Xusheng Qiu, Chan Ding, Ying Liao*. The Ubiquitin-Proteasome System facilitates membrane fusion and uncoating during coronavirus entry. Viruses. 2023, 15, 2001. (*Corresponding authors)
3) Wenlian Weng, Qingyan Liu, Wenxiang Xue, Huan Wang, Shouguo Fang, Yingjie Sun, Lei Tan, Cuiping Song, Xusheng Qiu, Weiwei Liu, Chan Ding, Ying Liao*. Characterization of the Protective Efficacy against QX Strain of a Recombinant Infectious Bronchitis Virus with H120 Backbone and QX Spike Gene. Frontiers in Microbiology. 2022. June 17, 883642. (*Corresponding authors)
4) Wenxiang Xue, Chan Ding, Kun Qian, Ying Liao*. The interplay between coronavirus and type I IFN response. Frontiers in Microbiology. 2022. March 04. 805472. (*Corresponding authors)
5) Bo Gao#, Xiaoqian Gong#, Shouguo Fang, Wenlian Weng, Huan Wang, Hongyan Chu, Yingjie Sun, Chunchun Meng, Lei Tan, Cuiping Song, Xusheng Qiu, Weiwei Liu, Maria Forlenza, Chan Ding, Ying Liao*. Inhibition of Anti-viral Stress Granule Formation by coronavirus endoribonuclease nsp15 Ensures Efficient Virus Replication. Plos Pathogens . 2021. Feb 26;17(2): e1008690 (*Corresponding authors)
6) Huan Wang, Dingxiang Liu, Yingjie Sun, Chunchun Meng, Lei Tan, Cuiping Song, Xusheng Qiu, Weiwei Liu, Chan Ding, Ying Liao*. Upregulation of DUSP6 impairs infectious bronchitis virus replication by negatively regulating ERK pathway and promoting apoptosis. Veterinary Research. 2021. Jan 11;52(1):7. (*Corresponding authors)
7) Yanrong Li#, Weiyu Jiang, Qiaona Niu, Yingjie Sun, Chunchun Meng, Lei Tan, Cuiping Song, Xusheng Qiu, Ying Liao*#, Chan Ding* eIF2α-CHOP-BCl2/JNK and IRE1α-XBP1/JNK Signaling Promote Apoptosis and Inflammation and Support the proliferation of Newcastle Disease Virus. Cell death & Disease s. 2019. Nov 26;10 (12):891. (*Corresponding authors)
8) Han S#, Mao L#, Liao Y#, Sun S, Zhang Z, Mo Y, Liu H, Zhi X, Lin S, Seo HS, Guo H. Sec62 Suppresses Foot-and-Mouth Disease Virus Proliferation by Promotion of IRE1α-RIG-I Antiviral Signaling. Journal of Immunology. 2019 Jul 15;203(2):429-440. (# Co-first authors)
9) Wang H, Yuan X, Sun Y, Mao X, Meng C, Tan L, Song C, Qiu X, Ding C, Liao Y*. Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system. Virology. 2019; 528:118-136. (*Corresponding authors)
10) Fan XX#, Han SC#, YanD, GaoY, WeiYQ, LiuXT, Liao Y*, Guo HC*, Sun SQ*. Foot-and–mouth disease virus infection suppresses autophagy and NF-кB antiviral responses via degradation of ATG5-ATG12 by 3Cpro Cell Death & Diseases. 2017. Jan 19;8(1): e2561. (*Co-corresponding authors)
11) Liao Y#*, Wang HX#, Mao X, Fang H, Wang H, Li Y, Sun Y, Meng C, Tan L, Song C, Qiu X, Ding C*. RIP1 is a central signaling protein in regulation of TNF-α/TRAIL mediated apoptosis and necroptosis during Newcastle disease virus infection. Oncotarget. 2017. 8 (26): 43201-43217. (# Co-first authors, *Co-corresponding authors)
12) Wang X, Wang R, Luo M, Li C, Wang HX, Huan CC, Qu YR, Liao Y*, Mao X*. (DEAD)-box RNA helicase 3 modulates NF-κB signal pathway by controlling the phosphorylation of PP2A-C subunit. Oncotarget. 2017 May16;8(20): 33197-33213. (*Co-corresponding authors)
13) Fung TS, Liao Y, Liu DX. Regulation of Stress Responses and Translational Control by Coronavirus. Viruses. 2016 Jul 4;8(7). pii: E184. doi: 10.3390/v8070184.
14) Liao Y#*, Gu F#, Mao X, Niu Q, Wang H, Sun Y, Song C, Qiu X, Tan L, Ding C*. 2016. Regulation of de novo Translation of Host Cells by Manipulation of PERK/PKR and GADD34-PP1 Activity during Newcastle Disease Virus Infection. J Gen Virol. 2016 Apr;97(4):867-79. (#Co-first authors, *Co-corresponding authors)
15) Liao Y,Zhang SM, Neo TL, Tam JP. 2015. Tryptophan-dependent membrane interaction and heteromerization with the internal fusion peptide by the membrane proximal external region of SARS-CoV spike protein. Biochemistry. 54(9):1819-30.
16) Liao Y,Fung TS, Huang M, Fang SG, Zhong YX, Liu DX.2014. RNA Isolation and Northern Blot Analysis. Bio-protocol. Vol 4, Iss 6
17) Liao Y, Fung TS, Huang M, Fang SG, Zhong Y, Liu DX. 2013. Up-regulation of CHOP/GADD153 during Coronavirus Infectious Bronchitis Virus Infection Modulates Apoptosis by Restricting Activation of the Extracellular Signal-Regulated Kinase Pathway. J Virol. 87(14):8124-34. May 15.
18) Liao Y,Wang X, Huang M, Tam JP, Liu DX. 2011. Regulation of the p38 mitogen-activated protein kinase and dual-specificity phosphatase 1 feedback loop modulates the induction of interleukin 6 and 8 in cells infected with coronavirus infectious bronchitis virus. Virology. 420 (2):106-16.
19) Wang, X.X.#, Liao,Y.#, Yap PL, Png KJ, Tam JP, Liu DX. Inhibition of protein kinase R activation and upregulation of GADD34 expression play a synergistic role in facilitating coronavirus replication by maintaining de novo protein synthesis in virus-infected cells. J Virol. 2 009. 83(23):12462-72. (#Co-first authors)
20) Liao, Y.,Yuan, Q., Torres, J., Tam, J. P., and Liu, D. X. 2006. Biochemical and functional characterization of the membrane association and membrane permeabilizing activity of the severe acute respiratory syndrome coronavirus envelope protein. Virology. 349:264-275.
21) Liao, Y., Lescar, J., Tam, P. J., and Liu, D. X. 2004. Expression of SARS-coronavirus envelope protein in Escherichia coli cells alters membrane permeability. BBRC . 325: 374-380.
22) Liao, Y., Tam, J. P., and Liu, D. X. 2005. Viroporin activity of SARS-CoV E protein. Adv. Exp. Med. Biol. 199-202.
7. Patent
A cell line expressing furin protein and its application in the culture of avian infectious bronchitis virus. Ying Liao, Ding Shan, Wang Huan, Sun Yingjie, Tan Lei, Meng Chunchun, Song Cuiping, Qiu Xusheng, and Liu Weiwei. Patent No.: zl 202011211170.6, authorization notice No.: cn 112342244b, authorization announcement date: June 30, 2023.