一、个人简历

单同领，博士，研究员，博士生导师，副主任，入选中国农业科学院所级青年英才、国家级人才、上海市领军人才“预备队”培养计划，获得上海市人才发展资金资助，中国农业科学院脱贫攻坚和乡村振兴先进个人。野生动物生物安全管控国家创新联盟副理事长，中国微生物学会兽医微生物学专业委员会青年委员，Frontiers in Veterinary Science、BMC Veterinary Research等杂志编委。

长期从事动物病毒组和冠状病毒的基础和应用研究；主持国家重点研发计划项目、课题和国家自然科学基金等10余项；在《Microbiome》、《Autophagy》（3篇）、《Science of the Total Environment》、《Emerging infection Disease》、《Journal of Virology》、《Journal of Biological Chemistry》等国际主流期刊发表SCI论文100余篇，其中第一或通信作者SCI论文60余篇，单篇被引270余次；获批兽医生物制品临床试验批件1项，授权发明专利4项，已经成果转化。培养研究生30余名，其中多名硕士研究生以第一作者在《Autophagy》、《Journal of Virology》、《Journal of Biological Chemistry》和《Microbiology Spectrum》等主流SCI期刊发表论文。

二、联系方式

邮   箱：shantongling@shvri.ac.cn

三、教育经历

2001.09-2005.07，河南农业大学，牧医工程学院，学士学位

2005.09-2008.06，华中农业大学，动医动科学院，硕士学位

2006.06-2008.01，中国农业科学院北京畜牧所联合培养

2008.09-2011.06，上海交通大学农业与生物学院，博士学位

2010.05-2011.05，美国加州大学旧金山分校访问学者

四、工作经历

2011.08-2013.12，中国农业科学院上海兽医研究所助理研究员

2014.01-2018.12，中国农业科学院上海兽医研究所副研究员

2019.01-至今，中国农业科学院上海兽医研究所 研究员

2020.08-2020.10，西藏自治区林芝市农业农村局 技术服务

2020.12-至今，中国农业科学院上海兽医研究所猪病室副主任

五、代表论著（*第一作者，#通信作者）

1. Xueying Zhai*, Ning Kong*, Yu Zhang*, Yiyi Song*, Wenzhen Qin* , Xinyu Yang, Chenqian Ye, Manqing Ye, Wu Tong, Changlong Liu, Hao Zheng, Hai Yu, Wen Zhang, Xia Yang, Gaiping Zhang ^#, Guangzhi Tong ^#, Tongling Shan ^#. N protein of PEDV plays chess game with host proteins by selective autophagy. Autophagy. 2023;1-15. doi: 10.1080/15548627.2023.2181615. Online ahead of print.

2. Tongling Shan *, Shixing Yang * , Haoning Wang *, Hao Wang* , Ju Zhang , Ga Gong, Yuqing Xiao, Jie Yang, Xiaolong Wang, Juan Lu, Min Zhao, Zijun Yang, Xiang Lu, Ziyuan Dai， Yumin He, Xu Chen, Rui Zhou, Yuxin Yao, Ning Kong, Jian Zeng, Xiaochun Wang, Quan Shen, Xutao Deng, Jianmin Zhang, Eric Delwart , Guangzhi Tong #, Wen Zhang#.Virome in cloaca of wild and breeding birds revealed diversity of significant viruses. Microbiome. 2022. 12;10(1):60. doi: 10.1186/s40168-022-01246-7.

3. Dage Sun^*, Ning Kong^*, Sujie Dong ^*, Xiaoyong Chen, Wenzhen Qin, Hua Wang, Yajuan Jiao, Huanjie Zhai, Liwei Li, Fei Gao, Lingxue Yu, Hao Zheng, Wu Tong, Hai Yu, Wen Zhang, Guangzhi Tong ^# and Tongling Shan ^#.2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8. Autophagy. 2022;18(8):1969-1981. doi: 10.1080/15548627.2021.2015740..

4. Ning Kong*, Tongling Shan*, Hua Wang, Yajuan Jiao , Yewen Zuo , Liwei Li, Wu Tong, Lingxue Yu, Yifeng Jiang , Yanjun Zhou , Guoxin Li, Fei Gao, Hai Yu , Hao Zheng , Guangzhi Tong#. BST2 Suppresses Porcine Epidemic Diarrhea Virus Replication by Targeting and Degrading Virus Nucleocapsid Protein With Selective Autophagy. Autophagy. 2020;16(10):1737-1752. doi: 10.1080/15548627.2019.1707487

5. Min Zhao*, Chanjuan Yue*, Zijun Yang, Yunli Li, Dongsheng Zhang, Ju Zhang, Shixing Yang, Quan Shen, Xiaoyan Su, Dunwu Qi, Rui Ma, Yuqing Xiao, Rong Hou, Xia Yan, Lin Li, Yanshan Zhou, Jiabin Liu, Xiaochun Wang, Wei Wu, Wen Zhang#, Tongling Shan#, Songrui Liu#.Viral metagenomics unveiled extensive communications of viruses within giant pandas and their associated organisms in the same ecosystem. Science of the Total Environment. 2022 Jan 20;153317. doi: 10.1016/j.scitotenv.2022.153317. Online ahead of print.

6. Xiaoquan Wang*, Wu Tong *, Xinyu Yang*, Huanjie Zhai*, Wenzhen Qin, Changlong Liu, Hao Zheng, Hai Yu, Guangzhi Tong Zhendong Zhang^#, Ning Kong^#, Tongling Shan ^#. RBM14 inhibits PEDV replication by recruiting P62 to degrade nuclear coat protein N through the autophagy pathway and promoting IFN expression. Journal of Virology, 2024 Mar 19;98(3):e0018224. doi: 10.1128/jvi.00182-24.

7. Xinyu Yang*, Ning Kong*, Wenzhen Qin*, Xueying Zhai, Yiyi Song, Wu Tong, Changlong Liu, Hao Zheng, Hai Yu, Wen Zhang#, Guangzhi Tong#, and Tongling Shan#. PGAM5 Degrades PDCoV N Protein and Activates Type-I Interferon to Antagonize Viral Replication. Journal of Virology, 2023 Oct 26:e0147023. doi: 10.1128/jvi.01470-23.

8. Wenzhen Qin^{a, b*}, Ning Kong^{a *}, Chunmei Wang ^{a *}, Sujie Dong^a, Huanjie Zhai^a, Xueying Zhai^a, Xinyu Yang^a, Chenqian Ye^a, Manqing Ye^a, Wu Tong^a, Changlong Liu^a, Lingxue Yu^a, Hao Zheng^a, Hai Yu^a, Daoliang Lan^b, Wen Zhang^c#, Guangzhi Tong^a#, Tongling Shan^a#. hnRNP K degrades viral nucleocapsid protein and induces type I IFN production to inhibit porcine epidemic diarrhea virus replication. Journal of Virology. 2022 Nov 1:e0155522. doi: 10.1128/jvi.01555-22. Online ahead of print.

9. Wenzhen Qin^{a, b*}, Ning Kong^{a *}, Yu Zhang^c*, Chunmei Wang ^{a *}, Sujie Dong^a, Huanjie Zhai^a, Xueying Zhai^a, Xinyu Yang^a, Chenqian Ye^a, Manqing Ye^a, Wu Tong^a, Changlong Liu^a, Lingxue Yu^a, Hao Zheng^a, Hai Yu^a, Wen Zhang^d, Daoliang Lan^b, Guangzhi Tong^a#, Tongling Shan^a#. PTBP1 suppresses porcine epidemic diarrhea virus replication via inducing protein degradation and IFN production. Journal of Biological Chemistry.  2023 Jun 29;299(8):104987. doi: 10.1016/j.jbc.2023.104987.

10. Xueying Zhai^{a, b*}, Ning Kong^{b *}, Chunmei Wang ^{b *}, Wenzhen Qin^b, Sujie Dong^b, Huanjie Zhai^b, Xinyu Yang^b, Chenqian Ye^b, Manqing Ye^b, Guoxin Li^b, Wu Tong^b, Changlong Liu^b, Hao Zheng^b, Hai Yu^b, Wen Zhang^c, Xia Yang ^a#, Guangzhi Tong^b#, Tongling Shan^b#. PRPF19 limits porcine epidemic diarrhea virus replication through targeting and degrading viral capsid protein. Journal of Virology. 2023 Jan 31;97(1):e0161422. doi: 10.1128/jvi.01614-22

11. Sujie Dong ^*, Ning Kong ^*, Chunmei Wang^*,Youwen Li, Dage Sun, Wenzhen Qin, Huanjie Zhai, Xueying Zhai, Xinyu Yang, Chenqian Ye, Manqing Ye, Changlong Liu, Lingxue Yu, Hao Zheng, Wu Tong, Hai Yu, Wen Zhang, Guangzhi Tong ^#, Tongling Shan ^#.FUBP3 degrades the PEDV nucleocapsid protein and induces the production of type I interferon. Journal of Virology. 2022 Jul 13;96(13):e0061822. doi: 10.1128/jvi.00618-22. Epub 2022 Jun 13

12. Wenzhen Qin*, Ning Kong *, Yu Zhang*, Sujie Dong, Huanjie Zhai, Xueying Zhai, Xinyu Yang, Chenqian Ye, Manqing Ye, Changlong Liu, Lingxue Yu, Hao Zheng, Hai Yu, Wen Zhang, Guangzhi Tong, Daoliang Lan^#, Wu Tong^#, Tongling Shan^#. Nuclear ribonucleoprotein RALY targets virus nucleocapsid protein and induces autophagy to restrict porcine epidemic diarrhea virus replication. Journal of Biological Chemistry. 2022 Aug;298(8):102190. doi: 10.1016/j.jbc.2022.102190. Epub 2022 Jun 24.

13. Sujie Dong *, Ning Kong *, Yu Zhang*, Youwen Li*, Dage Sun, Wenzhen Qin, Huanjie Zhai, Xueying Zhai, Xinyu Yang, Chenqian Ye, Manqing Ye, Changlong Liu, Lingxue Yu, Hao Zheng, Wu Tong, Hai Yu, Wen Zhang, Guangzhi Tong #, Tongling Shan #.TARDBP inhibits porcine epidemic diarrhea virus replication by degrading viral nucleocapsid protein and activating type I IFN signaling. Journal of Virology. 2022 May 25;96(10):e0007022. doi: 10.1128/jvi.00070-22. Epub 2022 May 2

14. Hua Wang*, Ning Kong*, Yajuan Jiao*, Sujie Dong*, Dage Sun, Xiaoyong Chen, Hao Zheng, Wu Tong, Hai Yu, Lingxue Yu, Wen Zhang, Guangzhi Tong#, Tongling Shan#.EGR1 suppresses porcine epidemic diarrhea virus replication by regulating IRAV to degrade viral nucleocapsid protein. Journal of Virology. 2021 Jul 21:JVI0064521. doi: 10.1128/JVI.00645-21. Epub ahead of print. PMID: 34287043.

15. Zijun Yang*, Hao Wang*, Shixing Yang, Xiaochun Wang, Quan Shen, Likai Ji, Jian Zeng, Wen Zhang^#, Haiyan Gong^#, Tongling Shan^#. Virome diversity of ticks feeding on domestic mammals in China. Virologica sinica. 2023 11;S1995-820X(23)00009-3. doi: 10.1016/j.virs.2023.02.001. Online ahead of print.

16. Ziyuan Dai*, Haoning Wang*, Haisheng Wu*, Qing Zhang, Likai Ji, Xiaochun Wang, Quan Shen, Shixing Yang, Xiao Ma, Tongling Shan#, Wen Zhang#. Parvovirus dark matter in the cloaca of wild birds. Gigascience. 2023;12:giad001. doi: 10.1093/gigascience/giad001.

17. Min Zhao*,, Siwen Bao*,, Diandian Xu, Jingxian He , Han Zhang , Likai Ji, Shixing Yang, Xiaochun Wang, Quan Shen, Jia Liu, Qing Zhang, Xiao Ma#, Wen Zhang #, Tongling Shan #. Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences. Virology Journal. 2023 Mar 9;20(1):46. doi: 10.1186/s12985-023-02005-2

18. Yajuan Jiao*, Ning Kong*, Hua Wang*, Dage Sun*, Sujie Dong, Xiaoyong Chen, Hao Zheng, Wu Tong, Hai Yu, Lingxue Yu, Yaowei Huang, Huan Wang, Baokun Sui, Ling Zhao, Ying Liao, Wen Zhang, Guangzhi Tong #, Tongling Shan#.PABPC4 broadly inhibits coronavirus replication by degrading nucleocapsid protein through selective autophagy. Microbiology Spectrum. 2021, 6: e0090821. doi: 10.1128/Spectrum.00908-21.

19. Yuan Xi*, Xiaojie Jiang*, Xinrui Xie*, Min Zhao, Han Zhang, Kailin Qin, Xiaochun Wang, Yuwei Liu, Shixing Yang, Quan Shen , Likai Ji , Peng Shang# , Wen Zhang# , Tongling Shan#. Viromics Reveals the High Diversity of Viruses from Fishes of the Tibet Highland. Microbiology Spectrum. 2023 Jun 15;11(3):e0094623. doi: 10.1128/spectrum.00946-23

20. Huanjie Zhai, Wenzhen Qin, Sujie Dong, Xinyu Yang, Xueying Zhai, Wu Tong, Changlong Liu, Hao Zheng, Hai Yu , Ning Kong, Guangzhi Tong, Tongling Shan. PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication. Veterinary Microbiology. 2023 Aug 9;284:109844.  doi: 10.1016/j.vetmic.2023.109844.

21. Shixing Yang*, Qingqing Mao*, Yan Wang , Jingxian He , Jie Yang , Xu Chen, Yuqing Xiao, Yumin He, Min Zhao, Juan Lu, Zijun Yang, Ziyuan Dai, Qi Liu, Yuxin Yao, Xiang Lu, Hong Li, Rui Zhou, Jian Zeng, Wang Li, Chenglin Zhou, Xiaochun Wang, Quan Shen, Hui Xu, Xutao Deng, Eric Delwart, Tongling Shan#, Wen Zhang#.Expanding known viral diversity in plants: virome of 161 species alongside an ancient canal. Environmental Microbiome.2022 Nov 27;17(1): 58.doi: 10.1186/s40793-022-00453-x.

22. Rui Ma*, Min Zhao*, Haoning Wang* , Rong Hou, Kailin Qin, Yu Qian, Han Zhang, Yanshan Zhou, Wei Wu, Jiang Gu, Xiaochun Wang, Quan Shen , Songrui Liu, Jiabin Liu, Wenlei Bi 1, Xiang Yu, Shixing Yang, Feifei Feng, Zusheng Li, Long Zhang, Guanwei Lan, Chao Chen, Fei Xue, Yan Wang, Huang Chong, Yang Hong, Likai Ji, Yuwei Liu, Dunwu Qi#, Tongling Shan#, Wen Zhang#.Virome of Giant Panda-Infesting Ticks Reveals Novel Bunyaviruses and Other Viruses That Are Genetically Close to Those from Giant Pandas. Microbiology Spectrum. 2022 Aug 2:e0203422

23. Juan Lu*, Shixing Yang*, Chunmei Wang*, Hao Wang*, Ga Gong , Yuan Xi , Jiamin Pan , Xiaochun Wang , Mr. Jiang Zeng , Ju Zhang , Peng Li , Prof. Quan Shen, Tongling Shan# , Wen Zhang#. Gut virome of the world's highest lizard species (Phrynocephalus erythrurus and Phrynocephalus theobaldi) reveals versatile commensal viruses. Microbiology Spectrum. 2022 Feb 23;10(1):e0187221

24. Juan Lu* , Haoning Wang*, Chunmei Wang*, Min Zhao , Rong Hou , Quan Shen , Shixing Yang , Likai Ji , Yuwei Liu, Xiaochun Wang, Songrui Liu , Tongling Shan#, Wen Zhang#. Gut phageome of the giant panda ( Ailuropoda melanoleuca) reveals greater diversity than relative species. mSystems. 2023 Jun 5; e0016123. msystems.00161-23.

25. Xiaojie Jiang*, Jia Liu*, Yuan Xi*, Qing Zhang, Yongshun Wang, Min Zhao, Xiang Lu, Haisheng Wu, Tongling Shan#, Bin Ni#, Wen Zhang#, Xiao Ma#. Virome of high-altitude canine digestive tract and genetic characterization of novel viruses potentially threatening human health. mSphere. 2023 Sep 19;e0034523. doi: 10.1128/msphere.00345-23

26. Shixing Yang*, Tongling Shan*, Yuqing Xiao*, Heteng Zhang, Xiaochun Wang, Quan Shen, Yan Wang, Yuxin Yao, Qi Liu, Hao Wang #, Wen Zhang#. Digging metagenomic data of pangolins revealed SARS-CoV-2 related viruses and other significant viruses. Journal of Medical Virology. 2021. doi: 10.1002/jmv.26524. Online ahead of print.

27. Yu Zhang*, Chunmei Wang*, Ce Zhu, Wei Ye, Qin Gu, Chenbin Shu, Xiping Feng, Xi Chen, Wen Zhang#, Tongling Shan#.Redondoviridae infection regulates circRNAome in periodontitis. Journal of Medical Virology. 2022 Jan 24. doi: 10.1002/jmv.27624.

28. Zi Zhuang, Lingling Qian, Juan Lu, Xiaodan Zhang, Asif Mahmood , Lei Cui, Huiying Wang, Xiaochun Wang, Shixing Yang, Likai Ji, Tongling Shan^#, Quan Shen^#, Wen Zhang^#.Comparison of viral communities in the blood, feces and various tissues of wild brown rats ( Rattus norvegicus). Heliyon. 2023 Jun 13;9(6):e17222.  doi: 10.1016/j.heliyon.2023.e17222. eCollection 2023 Jun.

29. Lingling Qian, Zi Zhuang, Juan Lu, Huiying Wang, Xiaochun Wang, Shixing Yang, Likai Ji, Quan Shen, Wen Zhang, Tongling Shan. Metagenomic survey of viral diversity obtained from feces of piglets with diarrhea. Heliyon. 2024

30. Jiamin Pan ^*, Likai Ji ^*, Haisheng Wu ^*, Xiaochun Wang, Yan Wang, Yan Wu, Shixing Yang, Quan Shen, Yuwei Liu, Wen Zhang^#, Keshan Zhang^#, Tongling Shan^#. Metagenomic analysis of herbivorous mammalian viral communities in the Northwest Plateau. BMC Genomics. 2023 Sep 25;24(1):568. doi: 10.1186/s12864-023-09646-1.

六、专利标准

1. 童光志，单同领，潘溪，孔宁，李国新，童武，郑浩，杨莘，抗猪流行性腹泻病毒N蛋白单克隆抗体的制备和应用，2019年3月29日，中国，ZL201410384478.9。

2. 童光志、杨莘、周艳君、姜一峰、单同领、童武、郑浩，猪SAMHD1基因、蛋白、单克隆抗体及其应用，2018年5月18日，中国， ZL201410028266.7。

3. 童光志、童武、郑浩、刘飞、梁超、周艳君、单同领、于海、姜一峰、高飞，伪狂犬病毒基因缺失弱毒株及其制备方法和应用，2018年9月7日，中国，ZL201410002656.7。

4. 童光志; 郑浩; 童武; 李国新; 周艳君; 于海; 单同领; 高飞; 姜一峰; 虞凌雪，表达猪瘟病毒E2蛋白的重组伪狂犬病毒毒株及其制备方法和应用，2018年6月19日，中国， CN201810627238.5。

七、奖励成果

1. 兽用生物制品临床试验批件，猪流行性腹泻病毒II型活疫苗（TBJS株）临床试验，批件号：2021004，批准日期：2021年4月14日

八、 承担科研项目

1. 国家重点研发计划项目，2023YFD1801300，濒危与青藏高原动物疫病防控技术研发与应用，2023.10-2027.12，1980万元，主持，在研

2. 中国农业科学院领军人才（B类）2022.9-2027.9，500万元，主持

3. 国家自然科学基金面上项目，32272999. 猪流行性腹泻病毒N蛋白挟持宿主蛋白翻译系统促进病毒增殖的分子机制. 2023.01-2026.12，54万元，主持，在研

4. 科技部，国家重点研发计划子课题，2021YFD1801102，畜禽冠状病毒的入侵与复制机制，2021/12-2024/12,175万元，在研

5. 国家自然科学基金面上项目，31872478，GSK3介导β-catenin和N蛋白调控猪流行性腹泻病毒复制的分子机制研究，2019/01-2022/12, 70.8万元，结题，主持

6. 科技部，国家重点研发计划课题，2017YFC1200201，重要疫源动物微生群落与未知新发病原的耐药致病性和适应性进化，2017/07-2020/06，590万元，在研，结题

7. 科技部，国家重点研发计划子课题，2016YFD0500103，猪冠状病毒性腹泻的免疫保护机制研究，2016/07-2020/12，140万，结题，主持子课题

8. 横向课题，猪场重要猪病的监测和防控，2021/11-2026/10,100万元，主持

9. 国家自然科学基金青年项目，31201915， 新发仔猪腹泻病因的筛选及鉴定，2013/01-2015/12，25万元，结题，主持

10. 上海市科技兴农重点攻关项目，沪农科攻字（2013）第3-6号，猪消化道病原口服疫苗研究，2013/10-2017/4，32万元，结题，主持子课题

11. 上海市科技兴农重点攻关项目，沪农科攻字（2013）第5-5号，上海地区仔猪腹泻病原生态学及防控技术研究，2013/11-2016/11，60万元，结题，主持子课题