Recently, researchers from the Prevention and Control of Porcine Viral Reproductive Disorders team of SHVRI have made significant progress in the development of anti-recombinant vaccine of porcine reproductive and respiratory syndrome virus (PRRSV) by rewiring of transcriptional regulatory sequences (TRS). The related research results have been published in mBio.
Background
Recombination is a significant factor driving the evolution of RNA viruses. The prevalence and variation of PRRSV in China has been increasing in complexity due to extensive interlineage recombination.
Research Progress
In this study, the researchers analyzed the prevalence and variation of PRRSV in China to clarify the major interlineage recombination patterns and successfully constructed and rescued the mutant virus (vA-TRSall) by rewiring the entire TRS circuit without changing the amino acid-coding sequence in the genome of the PRRSV live vaccine strain HuN4-F112. Recombination analysis in vitro and in vivo showed that the incidence rates of mutation breakpoints and recombination were effectively reduced. The above results have enriched the understanding of the critical role of TRS circuits in PRRSV recombination mechanisms and indicate a successful redesign that can endow PRRSV live vaccines with recombination-resistant capabilities. It is of great significance for clinical prevention and control of the disease.
Funding
This study was supported by the Innovation Program of Chinese Academy of Agricultural Sciences, National Natural Science Foundation of China, National Key Research and Development Program of China, and Natural Science Foundation of Shanghai.
Original Link
https://pubmed.ncbi.nlm.nih.gov/39714179/.
