The viruses and Swine diseases of reproductive failures

 

 

                 

 

Tong, Guang-zhi, PhD, Chief scientist
Email: gztong@shvri.ac.cn or tongguangzhi@caas.cn

 

Mission:
Molecular epidemiology, mechanisms of immune or pathogenesis of swine viruses associated with reproductive failures.

Research direction：
Porcine reproductive and respiratory syndrome virus;
Pseudorabies virus;
Japanese encephalitis virus;
Porcine epidemic diarrhea virus;
Developing genetic engineering marker vaccine against diseases associated with swine reproductive failures.

 

Team member:
Zhou, Yan-jun, PhD, Research professor
Li, Guo-xin, PhD, Research professor
Huang, Qin-feng, PhD, Research professor
Zheng, Hao, PhD, Associate research professor
Shan,Tong-ling, PhD, Associate research professor
Gao, Fei, PhD, Associate research professor
Jiang, Yi-feng, PhD, Research assistant
Tong, Wu, MS, Research assistant
Yang, Shen, PhD, Postdoctor
Yu, Ling-xue, PhD, Postdoctor
Tang, Ping, PhD, Postdoctor
Li, Li-wei, PhD, Postdoctor

 

Major publications
Sheng Yang, Guangzhi Tong*. Innate Immune Responses against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus. British Journal of Virology, 2015, accepted.
Kuan Zhao, Chao Ye, Xiao-Bo Chang, Cheng-Gang Jiang, Xue-Hui Cai, Guang-Zhi Tong, Zhi-Jun Tian, Mang Shi*, Tong-Qing An*. Importation and recombination are responsible for the latest emergence of high pathogenic PRRSV in China. Journal of Virology, 2015, accepted.
Hongliang Zhang, Liping Feng ,Chunxiao Liu, Jiazeng Chen, Chaoliang Leng ,Yun Bai, Jinmei Peng, Tongqing An, Xuehui Cai, Xufu Yang Zhijun Tian*, Guangzhi Tong*. Complete genome sequence of a classical swine fever virus isolate, named JSZL, belonging to a new subgenotype, 2.1d, China, 2014. Genome Annoucements-Viruses, 2015, accepted.
Jia-zeng Chen, Jin-mei Peng, Yun Bai, Qian Wang, Yimin Liu, Qiu-yue Zhang, Dan Chang, Wu-chao Zhang, Hong-yuan Zhao, Chao Ye, Tong-qing An, Xue-hui Cai, Zhi-jun Tian*, Guang-zhi Tong*. Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene. Veterinary Microbiology, 2015, 176:344-351.
Chao Ye†, Qing-Zhan Zhang†, Zhi-Jun Tian†, Hao Zheng, Kuan Zhao, Fei Liu, Jin-Chao Guo, Wu Tong, Cheng-Gang Jiang, Shu-Jie Wang, Mang Shi, Xiao-Bo Chang, Yi-Feng Jiang, Jin-Mei  Peng, Yan-Jun Zhou, Yan-Dong Tang, Ming-Xia Sun, Xue-Hui Cai, Tong-Qing An*, Guang-Zhi  Tong*. Genomic characterization of emerged pseudorabies virus in China reveals marked sequence divergence: evidence for existence of two major genotypes. Virology, 2015, 483:32-43.
Yi-feng Jiang#，Tian-qi Xia#，Yan-jun Zhou，Ling-xue Yu， Shen Yang， Qin-feng Huang, Li-wei Li，Fei Gao，Ze-hui Qu，Wu Tong，Guang-zhi Tong *. Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain. Veterinary Microbiology, 2015, 179:242-249.
Hongliang Zhang，Chaoliang Leng，Liping Feng，Hongyue Zhai，Jiazeng Chen，Chunxiao Liu， Yun Bai，Chao Ye，Jinmei Peng，Tongqing An，Yunchao Kan，Xuehui Cai，Zhijun Tian*，Guangzhi Tong*. A new subgenotype 2.1d isolates of classical swine fever virus, China, 2014. Infection, Genetics and Evolution, 2015, 34:94-105.
Yan Xiao, Tong-Qing An*, Zhi-Jun Tian, Tian-Chao Wei, Yi-Feng Jiang, Jin-Mei Peng, Yan-Jun Zhou, Xue-Hui Cai, Guang-Zhi Tong*. The gene expression profile of porcine alveolar macrophages infected with a highly pathogenic porcine reproductive and respiratory syndrome virus indicates overstimulation of the innate immune system by the virus. Archives of Virology, 2015, 160:649-662.
Lichang Sun, Yan Zhou, Runxia Liu, Yanhua Li, Fei Gao, Xiaomin Wang, Hongjie Fan, Shishan Yuan, Zuzhang Wei*, Guangzhi Tong*. Cysteine residues of the Porcine Reproductive and Respiratory Syndrome Virus ORF5a Protein are not essential for virus viability. Virus Research, 2015, 197:17-25.
Lingxue Yu, Yanjun Zhou, Yifeng Jiang, Wu Tong, Shen Yang, Fei Gao, Kang Wang, Liwei Li, Tianqi Xia, Qun Cheng, Guangzhi Tong*. Construction and in vitro Evaluation of a Recombinant Live Attenuated PRRSV Expressing GM-CSF. Virology Journal, 2014, 11:201.
Shen Yang, Tongling Shan, Yanjun Zhou, Yifeng Jiang, Wu Tong, Fei liu, Feng Wen, Qingzhan Zhang, Guangzhi Tong*. Molecular cloning and characterizations of porcine SAMHD1 and its roles in replicaiton of highly pathogenic porcine reproductive and respiratory syndrome virus. Developmental & Comparative Immunology, 2014, 47(2):234-246.
Liwei Li, Zuzhang Wei, Yanjun Zhou, Fei Gao, Yifeng Jiang, Lingxue Yu, Hao Zheng, Wu Tong, Shen Yang, Haihong Zheng, Tongling Shan, Fei Liu, Tianqi Xia, Guangzhi Tong*. Host miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by upregulating type I interferons. Virus Research, 2014, 195:86-94.
Jia-zeng Chen, Qian Wang, Yun Bai, Bin Wang, Hong-yuan Zhao, Zhi-jun Tian*, Jin-mei Peng, Tong-qing, An, Guang-zhi Tong*. Identification of two dominant linear epitopes on the GP3 protein of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). Research in Veterinary Science, 2014, 97:238-243.
Shen Yang, Yan-jun Zhou, Yuan Zhan, Ling-xue Yu, Yi-feng Jiang, Wu Tong, Guang-zhi Tong*. Development of monoclonal antibodies specifically recognizing the endogenous sterile alpha motif and HD domain 1 (SAMHD1) protein in porcine cell lines. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 2014, 33(5):344-349.
Chao-liang Leng, Zhi-jun Tian, Wu-chao Zhang, Hong-liang Zhang, Hong-yue Zhai, Tong-qing An, Jin-mei Peng, Chao Ye, Long Sun, Qian Wang, Yan Sun, Lin Li, Hong-yuan Zhao, Dan Chang, Xue-hui Cai, Gui-hong Zhang*, Guang-zhi Tong*. Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013. Veterinary Microbiology, 2014, 171:41-52.   
Yan-jun Zhou, Jian-ping Zhu, Ya-xin Wang, Ling-xue Yu, Hai Yu, Li-qian Zhu, Yi-feng Jiang,  Guang-zhi Tong*. Identification of differentially expressed proteins in porcine alveolar macrophages infected with virulent/attenuated strains of porcine reproductive and respiratory syndrome virus. PLOS ONE, 2014, 9(1)：e85767.
Guoxin Li, Xuyuan Gao, Yali Xiao, Shaoqiong Liu, Shan Peng, Xuesong Li, Ying Shi, Yuee Zhang, Lei Yu, Xiaogang Wu, Pixi Yan, Liping Yan, Qiaoyang Teng, GuangzhiTong*, ZejunLi*. Development of a live attenuated vaccine candidate against duck Tembusu viral disease. Virology, 2014, 450-451:233-242.
Tong-Qing An1, Jin-Mei Peng1, Zhi-Jun Tian1, Hong-Yuan Zhao, Na Li, Yi-Min Liu, Jia-Zeng Chen, Chao-Liang Leng, Yan Sun, Dan Chang, Guang-Zhi Tong*. Pseudorabies Virus Variant in Bartha-K61–Vaccinated Pigs, China, 2012. Emerging Infectious Diseases, 2013, 19(11):1749- 1755.
Ji-Hong Ma, Fu-Ru Yang, Hai Yu, Yan-Jun Zhou, Guo-Xin Li, Meng Huang, Feng Wen, Guangzhi Tong*. An M2e-based synthetic peptide vaccine for influenza A virus confers heterosubtypic protection from lethal virus challenge. Virology Journal, 2013, 10:227.
Yang Yin, Changlong Liu, Ping Liu, Huochun Yao, Zuzhang Wei, Jiaqi Lu, Guangzhi Tong*, Fei Gao*，Shishan Yuan. The Conserved Nucleotides in the Terminus of 3’UTR Region are Important for the Replication and Infectivity of Porcine Reproductive and Respiratory Syndrome Virus. Archives of Virology，2013，158:1719-1732.
Fei Gao, Jiaqi Lu, Zuzhang Wei, Guangzhi Tong*, Shishan Yuan*. Replacement of the Heterologous 5'Untranslated Region allows Preservation of the Fully Functional Activities of Type 2 Porcine Reproductive and Respiratory Syndrome Virus. Virology, 2013, 439(1):1-12.
Liqian Zhuξ, Shen Yangξ, Wu Tong, Jianping Zhu, Hai Yu, Yanjun Zhou , Robert B. Morrison, Guangzhi Tong*. The control of PI3K/Akt pathway by porcine reproductive and respiratory syndrome virus. Achives of Virology, 2013,158:1227-1234.
Lichang Sun, Yanhua Li, Runxia Liu, Xiaomin Wang, Fei Gao, Tao Lin, Ting Huang, Huochun Yao, Guangzhi Tong, Hongjie Fan*, Zuzhang Wei*, Shishan Yuan. Porcine Reproductive and Respiratory Syndrome Virus ORF5a Protein is Essential for Virus Viability. Virus Research, 2013, 171:178-185.
Tong-Qing An, Yi Zhang, Zhi-Jun Tian*, Chao-Liang Leng, Yong-Qian Yang, Jin-Mei Peng, Guang-Zhi Tong*. Expression of short peptide by an improved isocaudamer tandem repeat strategy. Protein & Peptide Letters, 2013, 20(7):808-812.
Xiaomin Wang, Lichang Sun, Yanhua Li, Tao Lin, Fei Gao, Huochun Yao, Kongwang He, Guangzhi Tong*, Zuzhang Wei*, Shishan Yuan. Development of a differentiable virus via a spontaneous deletion in the nsp2 region associated with cell adaptation of porcine reproductive and respiratory syndrome virus. Virus Research, 2013, 171:150-160.
Yan-jun Zhou, Yu-lu Wu, Jan-pin Zhu, Wu Tong, Hai Yu, Yi-feng Jiang, and Guang-zhi Tong*. Complete genome sequence of a virulent porcine epidemic diarrhea virus strain. Journal of Virology, 2012，86（24）：13862.
Tongling Shan, Chunmei Wang, Wu Tong, Hao Zheng, Xiuguo Hua, Shen Yang, Wen Zhang, Guangzhi Tong*. Complete Genome of a Novel Porcine Astrovirus. Journal of Virology, 2012，86（24）:13820-13821.
Meng-meng Wang, Yan-jun Zhou, Qing-zhan Zhang, Jun-wei Hou, Hai Yu，and Guang-zhi Tong*. Complete Genome Sequences of Highly Divergent Torque Teno Virus type II from Swine Herds. Journal of Virology, 2012，86（22）：12465.
Meng-meng Wang, Yan-jun Zhou, Qing-zhan Zhang, Jun-wei Hou, Hai Yu，and Guang-zhi Tong*. Complete Genome Sequences of Highly Divergent Torque Teno Virus type I from Swine Herds. Journal of Virology, 2012, 86（21）：11953.
Chao-liang Leng, Tong-qing An, Jia-zeng Chen, Da-qing Gong, Jin-mei Peng, Yong-qian Yang, Jiang Wu, Juan-juan Guo, Deng-yun Li, Yi Zhang, Zhen-xiang Meng, Yu-quan Wu, Zhi-jun Tian*, Guang-zhi Tong*. Highly pathogenic porcine reproductive and respiratory syndrome virus GP5 B antigenic region is not a neutralizing antigenic region. Veterinary Microbiology, 2012, 159:273-281.
Yan-zhao Xu, Yan-jun Zhou, Shan-rui Zhang, Wu Tong, Ling Li, Yi-feng Jiang, Guang-zhi Tong*. Identification of nonessential regions in nsp2 gene of an attenuated porcine reproductive and respiratory syndrome virus for replication in MARC-145 cell. Virology Journal, 2012, 9：141.
Zuzhang Wei, Tao Lin, Lichang Sun, Yanhua Li, Xiaoming Wang, Fei Gao, Runxia Liu, Chunyan Chen, Guangzhi Tong*, Shishan Yuan*. N-linked Glycosylation of GP5 of Porcine Reproductive And Respiratory Syndrome Virus Is Critically Important for Virus Replication in vivo. Journal of Virology, 2012, 86（18）：9941-9951.
Tao Lin, Xiangrui Li*, Huochun Yao, Zuzhang Wei, Feifei Tan, Runxia Liu, Lichang Sun, Rong Zhang, Wenliang Li, Jiaqi Lu, Guangzhi Tong* and Shishan Yuan. Use of reverse genetics to develop a novel marker porcine reproductive and respiratory syndrome virus. Virus Genes, 2012, 45(3):548-555.
Yan-zhao Xu, Yan-jun Zhou, Shan-rui Zhang, Yi-feng Jiang, Wu Tong，Hai Yu, Guang-zhi Tong*. Stable Expression of Foreign Gene in Nonessential Region of Nonstructural Protein 2 (nsp2) of Porcine Reproductive and Respiratory Syndrome Virus: Applications for Marker Vaccine Design. Veterinary Microbiology, 2012, 159:1-10.
Zuzhang Wei, Debin Tian, Lichang Sun, Tao Lin, Fei Gao, Runxia Liu, Jiaqi Lu, Guangzhi Tong#, Shishan Yuan#. Influence of N-linked glycosylation of minor proteins of porcine reproductive and respiratory syndrome virus on infectious virus recovery and receptor interaction. Virology, 2012, 429:1-11.
Li-qian Zhu, Yan-jun Zhou, Guang-zhi Tong[]. Porcine reproductive and respiratory syndrome virus suppresses interferon production. Acta Virologica, 2012, 56:3-9.
Rong Zhang, Chunyan Chen, Zhi Sun, Feifei Tan, Jinshan Zhuang, Debing Tian, Guangzhi Tong, Shishan Yuan*. Disulfide linkages mediating nucleocapsid protein dimerization are non-essential for porcine arterivirus particle formation. Journal of Virology, 2012, 86:4670-4681.
Debin Tian, Zuzhang Wei, Jessika C. Zevenhoven-Dobbe, Runxia Liu, Guangzhi Tong, Eric J. Snijder*, Shishan Yuan*. Arterivirus minor envelope proteins are a major determinant of viral tropism in cell culture. Journal of Virology, 2012，86:3701-3712.
Guo-Xin Li, Yan-Jun Zhou, Hai Yu, Ling Li, Ya-Xin Wang, Wu Tong, Jun-Wei Hou, Yan-Zhao Xu, Ao-Tian Xu, Guang-Zhi Tong*. A novel dendrimeric peptide induces high level neutralizing antibodies against classical swine fever virus in rabbits. Veterinary Microbiology, 2012, 156:200-204.
Shanrui Zhang, Yanjun Zhou, Yifeng Jiang, Guoxin Li, Liping Yan, Hai Yu, Guangzhi Tong*. Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus. Virology Journal, 2011, 8:410.
Tong-qing An, Zhi-jun Tian, Chao-liang Leng, Jin-mei Peng, Guang-zhi Tong*. Highly pathogenic porcine reproductive and respiratory syndrome virus, Asia. Emerging Infectious Diseases, 2011, 17(9):1782-1784.
Ya-Xin Wang, Yan-Jun Zhou, Guo-Xin Li, Shan-Rui Zhang, Yi-Feng Jiang, Ao-Tian Xu, Hai Yu, Meng-Meng Wang, Li-Ping Yan, Guang-Zhi Tong*. Identification of immunodominant T-cell eptitopes in membrane protein of the highly pathogenic porcine reproductive and respiratory syndrome virus. Virus Research, 2011, 158:108–115.
Tian-Chao Weia, Zhi-Jun Tiana, Yan-Jun Zhou, Tong-Qing An, Yi-feng Jiang, Yan Xiao, Shou-ping Hu, Jin-Mei Peng, Xiao-fang Hao, Shan-rui Zhang, Hai Yu, Guang-Zhi Tong*. Evaluation of the pathogenicity and immune response of a highly pathogenic porcine reproductive and respiratory syndrome virus variant to piglets. Agricultural Sciences in China, 2011, 10(8):1280-1291.
Tong-Qing An¶, Zhi-Jun Tian¶, Yan-Jun Zhou¶, Jin-Mei Peng, Jin Chen, Yan Xiao, Yi-Feng Jiang, Xiao-Fang Hao, Guang-Zhi Tong*. Comparative genomic analysis of five pairs of virulent parental/attenuated vaccine strains of porcine reproductive and respiratory syndrome virus: insight into the proteins potentially associated with attenuation. Veterinary Microbiology, 2011, 149:104-112.
Ao-Tian Xu ,Yan-Jun Zhou, Guo-Xin Li, Hai Yu, Li-Ping Yan, Guang-Zhi Tong*. Characterization of the biochemical properties and identification of amino acids forming the catalytic center of 3C-like proteinase of porcine reproductive and respiratory syndrome virus. Biotechnology Letters, 2010, 32:1905–1910.
Jin-mei Peng, Zhi-jun Tian, Heng-gui Liu, Tong-qing An, Yan-jun Zhou, Yao Wang, Guang-zhi Tong*. Cloning and identification of porcine programmed death 1(PD-1). Veterinary Immunology and Immunopathology, 2010, 136:157–162.
Tong-Qing An, Zhi-Jun Tian, Yun-Xia He, Yan Xiao, Yi-Feng Jiang, Jin-Mei Peng, Yan-Jun Zhou, Di Liu and Guang-Zhi Tong*. Porcine reproductive and respiratory syndrome virus attachment is mediated by the N-terminal domain of the sialoadhesin receptor. Veterinary Microbiology, 2010, 143: 371–378.
Tong-Qing An, Zhi-Jun Tian, Yan Xiao, Yan-Jun Zhou, Ran Li, Jin-Mei Peng, Tian-Chao Wei, Yi-Feng Jiang, Guang-Zhi Tong*. Origin of highly pathogenic porcine reproductive and respiratory syndrome virus, China. Emerging Infectious Diseases, 2010, 16(2):365-367.
Guo-Xin Li, Yan-Jun Zhou, Hai Yu, Zhi-Jun Tian, Li-ping Yan, Qiang Zhang, Shou-Ping Hu, Guang-Zhi Tong*. Prime-boost immunization with HA/C3d DNA followed by a recombinant pseudorabies virus boost enhanced protective immunity against H3N2 swine influenza virus in mice. Research in Veterinary Science, 2010, 88:345-351.
Yan-Jun Zhou¶, Hai Yu¶, Zhi-Jun Tian¶, Guo-Xin Li, Xiao-Fang Hao, Li-Ping Yan, Jin-Mei Peng, Ao-Tian Xu, Ya-Xin Wang, Tong-Qing An, Xue-Hui Cai, Li Feng, Xi Li, Guang-Zhi Tong*. Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008. Virus Research, 2009, 144:136-144.
Zhi-Jun Tian¶, Tong-Qing An¶, Yan-Jun Zhou¶, Jin-Mei Peng, Shou-Ping Hu, Tian-Chao Wei, Yi-Feng Jiang, Guang-Zhi Tong*. An attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) protects piglets against HP-PRRS. Veterinary Microbiology, 2009, 138:34-40.
Yan-Jun Zhou, Tong-Qing An, Jin-Xia Liu, Zhi-Jun Tian, Qiang Xue, Jin-Mei Peng, Yun-Feng Wang, Xue-Hui Cai, Guo-Xin Li, Mei Wang, Guang-Zhi Tong*. Monoclonal antibodies and conserved antigenic epitopes in the C terminus of GP5 protein of the North American type porcine reproductive and respiratory syndrome virus. Veterinary Microbiology, 2009, 138:1-10.
Bin Wanga, Rong-Hong Huaa, Zhi-Jun Tian, Na-Sha Chen, Fu-Rong Zhao, Tian-Qiang Liu, Yun-Feng Wang, Guang-Zhi Tong*. Identification of a Virus-specific and Conserved B-cell Epitope on NS1 Protein of Japanese Encephalitis Virus. Virus Research, 2009, 141:90-95.
Guo-Xin Li, Zhi-Jun Tian, Yuan-Yuan Jin, Shao-Hua Hou, Hai Yu, Tian-Qiang Liu, Shou-Ping Hu, Guang-Zhi Tong*. Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus. Research in Veterinary Science, 2009,86:406-413.
Yan-Jun Zhou, Xiao-Fang Hao, Zhi-Jun Tian, Guang-Zhi Tong*, Dongwan Yoo, Tong-Qing An, Hua-Ji Qiu, Tian-Chao Wei, Xiu-Fang Yuan. Highly virulent porcine reproductive and respiratory syndrome virus emerged in China. Transdoundary and Emerging Diseases, 2008, 55:152-164.
Wen-Bao Qi, Rong-Hong Hua, Li-Ping Yan, Guang-Zhi Tong*, Gui-Hong Zhang, Tao Ren, Dong-Lai Wu, Ming Liao*. Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Virus Research, 2008, 132(1-2):145-151.